Product Details
SoluProt: prediction of soluble protein expression in Escherichia coli
Created: 2021
Czech title
SoluProt: predikce rozpustné exprese proteinů v Escherichia coli
Type
software
License
Use of the result by another entity is possible without acquiring a license in some cases
License Fee
The licensor does not require a license fee for the result
Authors
Hon Jiří, Ing., Ph.D.
Marušiak Martin, Ing.
Martínek Tomáš, doc. Ing., Ph.D. (DCSY)
Kunka Antonín, Mgr., Ph.D.
Zendulka Jaroslav, doc. Ing., CSc. (UIFS)
Bednář David
Damborský Jiří, prof. Mgr., Dr. (UMEL)
Marušiak Martin, Ing.
Martínek Tomáš, doc. Ing., Ph.D. (DCSY)
Kunka Antonín, Mgr., Ph.D.
Zendulka Jaroslav, doc. Ing., CSc. (UIFS)
Bednář David
Damborský Jiří, prof. Mgr., Dr. (UMEL)
Keywords
protein solubility, machine-learning
Description
A new tool for sequence-based prediction of soluble protein expression in
Escherichia coli, SoluProt, was created using the gradient boosting machine
technique with the TargetTrack database as a training set. When evaluated against
a balanced independent test set derived from the NESG database, SoluProts
accuracy of 58.4% and AUC of 0.60 exceeded those of a suite of alternative
solubility prediction tools. There is also evidence that it could significantly
increase the success rate of experimental protein studies. SoluProt is freely
available as a standalone program and a user-friendly webserver at
https://loschmidt.chemi.muni.cz/soluprot/.
Location
Projects
Metody AI pro zabezpečení kybernetického prostoru a řídicí systémy, BUT, Vnitřní projekty VUT, FIT-S-20-6293, start: 2020-03-01, end: 2023-02-28, completed
Departments