Publication Details

CalFitter: a web server for analysis of protein thermal denaturation data

DAMBORSKÝ, J.; BEDNÁŘ, D.; KUNKA, A.; MAZURENKO, S.; NEDELJKOVIĆ, S.; PROKOP, Z.; ŠTOURAČ, J. CalFitter: a web server for analysis of protein thermal denaturation data. Nucleic Acids Research, 2018, vol. 46, no. 1, p. 344-349. ISSN: 1362-4962.
Czech title
CalFitter: webový server pro analýzu dat tepelné denaturace proteinů
Type
journal article
Language
English
Authors
Damborský Jiří, prof. Mgr., Dr. (UMEL)
Bednář David (FIT)
Kunka Antonín, Mgr., Ph.D.
Mazurenko Stanislav, Ph.D.
Nedeljković Sava, Bc. (VZ SC@FIT)
Prokop Zbyněk (FIT)
Štourač Jan (FIT)
URL
Keywords

Computational Bilology, Protein Denaturation, Internet, Protein Unfolding, Software

Abstract

Despite significant advances in the understanding of protein structure-function relationships, revealing protein folding pathways still poses a challenge due to a limited number of relevant experimental tools. Widely-used experimental techniques, such as calorimetry or spectroscopy, critically depend on a proper data analysis. Currently, there are only separate data analysis tools available for each type of experiment with a limited model selection. To address this problem, we have developed the CalFitter web server to be a unified platform for comprehensive data fitting and analysis of protein thermal denaturation data. The server allows simultaneous global data fitting using any combination of input data types and offers twelve protein unfolding pathway models for selection, including irreversible transitions often missing from other tools. The data fitting produces optimal parameters, their confidence intervals, and statistical information to define unfolding pathways. The server provides an interactive and easy-to-use interface that allows users to directly analyse input datasets and simulate modelled output based on the model parameters. CalFitter web server is available free at https://loschmidt.chemi.muni.cz/calfitter/.

Published
2018
Pages
344–349
Journal
Nucleic Acids Research, vol. 46, no. 1, ISSN 1362-4962
DOI
10.1093/nar/gky358
UT WoS
000438374100055
EID Scopus
2-s2.0-85050893810
BibTeX 
@article{BUT170435,
  author="Jiří {Damborský} and David {Bednář} and Antonín {Kunka} and Stanislav {Mazurenko} and Sava {Nedeljković} and Zbyněk {Prokop} and Jan {Štourač}",
  title="CalFitter: a web server for analysis of protein thermal denaturation data",
  journal="Nucleic Acids Research",
  year="2018",
  volume="46",
  number="1",
  pages="344--349",
  doi="10.1093/nar/gky358",
  issn="1362-4962",
  url="https://academic.oup.com/nar/article/46/W1/W344/4995690"
}
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