Publication Details

Deep Insights into the Specific Evolution of Fungal Hybrid B Heme Peroxidases

ZÁMOCKÝ, M.; MUSIL, M.; BANCHENKO, M.; FERIANC, P.; CHOVANOVÁ, K.; BARÁTH, P.; POLJOVKA, A.; BEDNÁŘ, D. Deep Insights into the Specific Evolution of Fungal Hybrid B Heme Peroxidases. Biology, 2022, vol. 11, no. 3, p. 1-18. ISSN: 2079-7737.
Czech title
Náhled do specifického vývoje houbových hybridních B hemperoxidáz
Type
journal article
Language
English
Authors
Zámocký Marcel
Musil Miloš, Ing., Ph.D. (DIFS)
Banchenko Maksym
Ferianc Peter
Chovanová Katarína
Baráth Peter
Poljovka Andrej
Bednář David
URL
Keywords

hybrid B heme peroxidase, peroxidasecatalase superfamily, oxidative
stress, enzymatic antioxidant, ancestral sequence reconstruction

Abstract

In this study, we focus on a detailed bioinformatics analysis of hyBpox genes,
mainly within the genomes of Sclerotiniaceae (Ascomycota, Leotiomycetes), which
is a specifically evolved fungal family of necrotrophic host generalists and
saprophytic or biotrophic host specialists. Members of the
genus Sclerotium produce only sclerotia and no fruiting bodies or spores. Thus,
their physiological role for peroxidases remains open. A representative
species, S. cepivorum, is a dangerous plant pathogen causing white rot
in Allium species, particularly in onions, leeks, and garlic. On a worldwide
basis, the white rot caused by this soil-borne fungus is apparently the most
serious threat to Allium-crop production. We have also found very similar
peroxidase sequences in the related fungus S. sclerotiorum, although with minor
yet important modifications in the architecture of its active centre. The
presence of ScephyBpox1-specific mRNA was confirmed by transcriptomic analysis.
The presence of Hybrid B peroxidase at the protein level as the sole
extracellular peroxidase of this fungus was confirmed in the secretome of S.
cepivorum through detailed proteomic analyses. This prompted us to systematically
search for all available genes coding for Hybrid B heme peroxidases in the whole
fungal family of Sclerotiniaceae. We present here a reconstruction of their
molecular phylogeny and analyse the unique aspects of their conserved-sequence
features and structural folds in corresponding ancestral sequences.

Published
2022
Pages
1–18
Journal
Biology, vol. 11, no. 3, ISSN 2079-7737
DOI
10.3390/biology11030459
UT WoS
000776175200001
EID Scopus
2-s2.0-85127589607
BibTeX 
@article{BUT177747,
  author="Marcel {Zámocký} and Miloš {Musil} and Maksym {Banchenko} and Peter {Ferianc} and Katarína {Chovanová} and Peter {Baráth} and Andrej {Poljovka} and David {Bednář}",
  title="Deep Insights into the Specific Evolution of Fungal Hybrid B Heme Peroxidases",
  journal="Biology",
  year="2022",
  volume="11",
  number="3",
  pages="1--18",
  doi="10.3390/biology11030459",
  issn="2079-7737",
  url="https://www.mdpi.com/2079-7737/11/3/459"
}
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