Publication Details

Fundamentally different repetitive element composition of sex chromosomes in Rumex acetosa

JESIONEK, W.; BODLÁKOVÁ, M.; KUBÁT, Z.; ČEGAN, R.; VYSKOT, B.; VRÁNA, J.; ŠAFÁŘ, J.; PUTEROVÁ, J.; HOBZA, R. Fundamentally different repetitive element composition of sex chromosomes in Rumex acetosa. Annals of Botany, 2021, vol. 127, no. 1, p. 33-47. ISSN: 1095-8290.
Czech title
Zásadně odlišné složení repetitivních sekvencí pohlavních chromozomů v Rumex acetosa
Type
journal article
Language
English
Authors
Jesionek Wojciech
BODLÁKOVÁ, M.
Kubát Zdeněk
ČEGAN, R.
Vyskot Boris, prof. RNDr., DrSc.
VRÁNA, J.
ŠAFÁŘ, J.
Puterová Janka, Ing., Ph.D. (FIT)
Hobza Roman
URL
Keywords

sex chromosomes, genome dynamics, transposable elements, satellites

Abstract

Dioecious species with well-established sex chromosomes are rare in the plant
kingdom. Most sex chromosomes increased in size but no comprehensive analysis of
the kind of sequences which drive this expansion has been presented. Here, we
analysed sex chromosome structure in common sorrel (Rumex acetosa), a dioecious
plant with XY1Y2 sex determination, and we provide the first chromosome specific
repeatome analysis for a plant species possessing sex chromosomes. We flow-sorted
and separately sequenced sex chromosomes and autosomes in R. acetosa using the
two-dimensional Fluorescence In Situ Hybridization in Suspension (FISHIS) method
and Illumina sequencing. We identified and quantified individual repeats using
RepeatExplorer, Tandem Repeat Finder and Tandem Repeats Analysis Program. We
employed Fluorescence In Situ Hybridization (FISH) to analyse chromosome
localisation of satellites and transposons. We identified a number of novel
satellites, which have, similarly as previously known satellites, significantly
expanded on the Y chromosome but not as much on the X nor on autosomes.
Additionally, the size increase of Y chromosomes is caused by non-LTR and LTR
retrotransposons, while only the latter ones contribute to the enlargement of the
X chromosome. However, the X chromosome is populated by different LTR
retrotransposon lineages than Y chromosomes. The X and Y chromosomes
significantly diverged in terms of repeat composition. The destiny of the
Y chromosomes has been shaped by a lack of recombination resulting in the
expansion of diverse satellites and microsatellites and faster fixation of newly
inserted transposable elements (TEs). In addition, X and Y chromosomes, despite
similar total counts of TEs, significantly differ in representation of individual
TE lineages, which indicates that transposons proliferate preferentially either
in the paternal or maternal lineage.

Published
2021
Pages
33–47
Journal
Annals of Botany, vol. 127, no. 1, ISSN 1095-8290
DOI
UT WoS
000646257300005
EID Scopus
BibTeX
@article{BUT168153,
  author="JESIONEK, W. and BODLÁKOVÁ, M. and KUBÁT, Z. and ČEGAN, R. and VYSKOT, B. and VRÁNA, J. and ŠAFÁŘ, J. and PUTEROVÁ, J. and HOBZA, R.",
  title="Fundamentally different repetitive element composition of sex chromosomes in Rumex acetosa",
  journal="Annals of Botany",
  year="2021",
  volume="127",
  number="1",
  pages="33--47",
  doi="10.1093/aob/mcaa160",
  issn="1095-8290",
  url="https://academic.oup.com/aob/article/127/1/33/5903355"
}
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