Publication Details

FireProt: web server for automated design of thermostable proteins

MUSIL, M.; ŠTOURAČ, J.; BENDL, J.; BREZOVSKÝ, J.; PROKOP, Z.; ZENDULKA, J.; MARTÍNEK, T.; BEDNÁŘ, D.; DAMBORSKÝ, J. FireProt: web server for automated design of thermostable proteins. Nucleic Acids Research, 2017, vol. 45, no. 1, p. 393-399. ISSN: 1362-4962.
Czech title
FireProt: web server pro automatizovaný návrh termostabilních proteinů
Type
journal article
Language
English
Authors
Musil Miloš, Ing., Ph.D. (DIFS)
Štourač Jan (FIT)
Bendl Jaroslav, Ing., Ph.D.
Brezovský Jan (FIT)
Prokop Zbyněk (FIT)
Zendulka Jaroslav, doc. Ing., CSc. (UIFS)
Martínek Tomáš, doc. Ing., Ph.D. (DCSY)
Bednář David (FIT)
Damborský Jiří, prof. Mgr., Dr. (UMEL)
URL
Keywords

mutations, protein engineering, mutant proteins, protein stability, thermostable proteins

Abstract

There is a continuous interest in increasing proteins stability to enhance their usability in numerous biomedical and biotechnological applications. A number of in silico tools for the prediction of the effect of mutations on protein stability have been developed recently. However, only single-point mutations with a small effect on protein stability are typically predicted with the existing tools and have to be followed by laborious protein expression, purification, and characterization. Here, we present FireProt, a web server for the automated design of multiplepoint thermostable mutant proteins that combines structural and evolutionary information in its calculation core. FireProt utilizes sixteen tools and three protein engineering strategies for making reliable protein designs. The server is complemented with interactive, easy-to-use interface that allows users to directly analyze and optionally modify designed thermostable mutants. FireProt is freely available at http://loschmidt.chemi.muni.cz/fireprot.

Published
2017
Pages
393–399
Journal
Nucleic Acids Research, vol. 45, no. 1, ISSN 1362-4962
DOI
10.1093/nar/gkx285
UT WoS
000404427000059
EID Scopus
2-s2.0-85023195078
BibTeX 
@article{BUT144434,
  author="Miloš {Musil} and Jan {Štourač} and Jaroslav {Bendl} and Jan {Brezovský} and Zbyněk {Prokop} and Jaroslav {Zendulka} and Tomáš {Martínek} and David {Bednář} and Jiří {Damborský}",
  title="FireProt: web server for automated design of thermostable proteins",
  journal="Nucleic Acids Research",
  year="2017",
  volume="45",
  number="1",
  pages="393--399",
  doi="10.1093/nar/gkx285",
  issn="1362-4962",
  url="https://doi.org/10.1093/nar/gkx285"
}
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