Publication Details
HotSpot Wizard 2.0: Web Server for Automated Design of Mutations and Smart Libraries
Štourač Jan
Chovancová Eva, Mgr., Ph.D.
Vávra Ondřej
Musil Miloš, Ing., Ph.D. (DIFS)
Brezovský Jan
Damborský Jiří, prof. Mgr., Dr. (UMEL)
protein engineering, smart library design, computational mutagenesis, hotspot
prediction, semi-rational enzyme design
HotSpot Wizard 2.0 is a web server for an automatic identification of hot spots
and design of mutations for engineering of enzyme activity, substrate
specificity, and enantioselectivity or protein stability. The server integrates
sequence, structural, and evolutionary information obtained from three databases
and nineteen computational tools to guide the users through the process of hot
spot selection by four protein engineering strategies as well as the optimization
of library size by narrowing down a set of substitutions at individual randomized
positions. The protein structure represents the only obligatory input for the
calculation. The results of calculations are mapped on the protein structure and
visualized in JSMol applet. For each implemented strategy, HotSpot Wizard lists
annotated residues suitable for mutagenesis and enables to design appropriate
codons. Altogether, HotSpot Wizard provides a comprehensive annotation of protein
structures and assists protein engineers with the design of mutations in
site-directed mutagenesis and focused directed evolution experiments. HotSpot
Wizard is freely available at http://loschmidt.chemi.muni.cz/hotspotwizard2.
@article{BUT133492,
author="Jaroslav {Bendl} and Jan {Štourač} and Eva {Chovancová} and Ondřej {Vávra} and Miloš {Musil} and Jan {Brezovský} and Jiří {Damborský}",
title="HotSpot Wizard 2.0: Web Server for Automated Design of Mutations and Smart Libraries",
journal="Nucleic Acids Research",
year="2016",
volume="44",
number="1",
pages="479--487",
doi="10.1093/nar/gkp410",
issn="1362-4962",
url="http://nar.oxfordjournals.org/content/early/2016/05/12/nar.gkw416.full"
}