Publication Details
Microbiome analysis and predicted relative metabolomic turnover suggest bacterial heme and selenium metabolism are altered in the gastrointestinal system of zebrafish (Danio rerio) exposed to the organochlorine dieldrin
BOYDA, J.
CRAFT, S.
GINN, P.
HUA, Q.
KOZUCH, M.
SCHMIDT, J.
Smatana Stanislav, Ing.
VESPALCOVÁ, H.
PERSICO, M.
BISESI, J.
BUDINSKÁ, E.
MARTYNIUK, C.
Microbiome, Pesticide, Aquatic, Ecotoxicology, Pathology
Dietary exposure to chemicals can alter the diversity of microbiome communities
and can lead to pathophysiological changes in the gastrointestinal system. The
organochlorine pesticide dieldrin is a persistent environmental contaminant that
bioaccumulates in fatty tissue of aquatic organisms. The objectives of this study
were to determine whether environmentally-relevant doses of dieldrin altered
gastrointestinal morphology and the microbiome of zebrafish. Adult zebrafish at
~4 months of age were fed a measured amount of feed containing either a solvent
control or one of two doses of dieldrin (measured at 16, and 163.5 ng/g dry
weight) for 4 months. Body burden levels of dieldrin in zebrafish at the end of
the four-month exposure were 0.600.52 ng/g (background), 11.471.13 ng/g (low
dose) and 18.321.32 ng/g (high dose) wet weight [meanstd]. Extensive
histopathology at the whole organism level revealed that dieldrin exposure did
not induce notable tissue pathology, including the gastrointestinal tract.
A repeated measures mixed model revealed that, while fish gained weight over
time, there were no dieldrin-specific effects on body mass. Fecal content was
collected from the gastrointestinal tract of males and 16S rRNA sequencing
conducted. Dieldrin at a dose of 16 ng/g reduced the abundance of Firmicutes, a
phylum involved in energy resorption. At the level of class, there was a decrease
in abundance of Clostridia and Betaproteobacteria, and an increase in
Verrucomicrobiae species. We used predicted relative metabolomic turnover (PRMA)
to predict how a shift in microbial community composition affects exchange of
metabolites. Dieldrin was predicted to affect metabolic turnover of
uroporphyrinogen I and coproporphyrinogen I [enzyme]cysteine, hydrogen selenide,
selenite, and methyl-selenic acid in fish. These pathways are related to
bacterial heme biosynthesis and selenium metabolism. Our study demonstrates that
dietary exposures to dieldrin can alter microbiota composition, however the
long-term consequences of such impacts are not well understood.
@article{BUT168159,
author="ADAMOVSKÝ, O. and BOYDA, J. and CRAFT, S. and GINN, P. and HUA, Q. and KOZUCH, M. and SCHMIDT, J. and SMATANA, S. and VESPALCOVÁ, H. and PERSICO, M. and BISESI, J. and BUDINSKÁ, E. and MARTYNIUK, C.",
title="Microbiome analysis and predicted relative metabolomic turnover suggest bacterial heme and selenium metabolism are altered in the gastrointestinal system of zebrafish (Danio rerio) exposed to the organochlorine dieldrin",
journal="ENVIRONMENTAL POLLUTION",
year="2020",
volume="2021",
number="268",
pages="115715--115726",
doi="10.1016/j.envpol.2020.115715",
issn="1873-6424",
url="https://www.sciencedirect.com/science/article/pii/S0269749120364046?casa_token=D-NvNb5oSOkAAAAA:TSekabQnMmwF-daj6ik3X3S1GHcD0DJnPcXtWzs4_hHCGfEXh0ZMGU1t_8Wyome1iZMN2kVeqw"
}