Publication Details
Evaluation of microbiome-host relationships in the zebrafish gastrointestinal system reveals adaptive immunity is a target of bis(2-ethylhexyl) phthalate (DEHP) exposure
BUERGER, A.
VESPALCOVÁ, H.
SOHAG, S.
HANLON, A.
GINN, P.
CRAFT, S.
Smatana Stanislav, Ing.
BUDINSKÁ, E.
PERSICO, M.
BISESI, J.
MARTYNIUK, C.
phthalate, DEHP, immunity, microbiome, gastrointestinal system
To improve physical characteristics of plastics such as flexibility and
durability, producers enrich materials with phthalates such as di-2-(ethylhexyl)
phthalate (DEHP). DEHP is a high production volume chemical associated with
metabolic and immune disruption in animals and humans. To reveal mechanisms
implicated in phthalate-related disruption in the gastrointestinal system, male
and female zebrafish were fed DEHP (3 ppm) daily for two months. At the
transcriptome level, DEHP significantly upregulated gene networks in the
intestine associated with helper T cells (Th1, Th2 and Th17) specific pathways.
The activation of gene networks associated with adaptive immunity were linked to
the suppression of networks for tight junction, gap junctional intercellular
communication, and transmembrane transporters, all of which are precursors for
impaired gut integrity and performance. On a class level, DEHP exposure
increased Bacteroidia and Gammaproteobacteria and decreased Verrucomicrobiae in
both the male and female gastrointestinal system. Further, in males there was
a relative increase in Fusobacteriia, Betaproteobacteria and a relative decrease
in Saccharibacteria. Predictive algorithms revealed the functional shift in the
microbiome community, and the metabolites they produce, act to modulate
intestinal adaptive immunity. This finding suggests that the gut microbiota may
contribute to the adverse effects of DEHP on the host by altering metabolites
sensed by both intestinal and immune Th cells. Our results suggest that the
microbiome-gut-immune axis can be modified by DEHP and emphasizes the value of
multi-omics approaches to study microbiome-host interactions following chemical
perturbations.
@article{BUT168158,
author="ADAMOVSKÝ, O. and BUERGER, A. and VESPALCOVÁ, H. and SOHAG, S. and HANLON, A. and GINN, P. and CRAFT, S. and SMATANA, S. and BUDINSKÁ, E. and PERSICO, M. and BISESI, J. and MARTYNIUK, C.",
title="Evaluation of microbiome-host relationships in the zebrafish gastrointestinal system reveals adaptive immunity is a target of bis(2-ethylhexyl) phthalate (DEHP) exposure",
journal="Environmental Science and Technology",
year="2020",
volume="54",
number="9",
pages="5719--5728",
doi="10.1021/acs.est.0c00628",
issn="1520-5851",
url="https://www.fit.vut.cz/research/publication/12232/"
}