FireProt 2.0

FireProt 2.0

software

Use of the result by another entity is possible without acquiring a license in some cases

The licensor does not require a license fee for the result

Musil Miloš, Ing., Ph.D. (DIFS)
Ježík Andrej, Bc.
Horáčková Jana, Mgr.
Borko Simeon, Ing.
Kabourek Petr
Damborský Jiří, prof. Mgr., Dr. (UMEL)
Bednář David

protein engineering, thermostable proteins, multiple-point mutants, protein
stability

FireProt protocol enables the automated computational design of highly stable
multiple-point mutants. FireProt 2.0 builds on top of the previously published
FireProt web, retaining the original functionality and expanding it with several
new stabilization strategies. FireProt 2.0 integrates the AlphaFold database and
the homology modelling for structure prediction, enabling calculations starting
from a sequence. Multiple-point designs are constructed using the Bron-Kerbosch
algorithm minimizing the antagonistic effect between the individual mutations.
Users can newly limit the FireProt calculation to a set of user-defined
mutations, run a saturation mutagenesis of the whole protein, or select
rigidifying mutations based on B-factors. Evolution-based back-to-consensus
strategy is complemented by ancestral sequence reconstruction. FireProt 2.0 is
significantly faster and a reworked graphical user interface broadens the tool's
availability even to users with older hardware.

Nástroj je dostupný na internetové adrese: http://loschmidt.chemi.muni.cz/fireprot

Chytré informační technologie pro odolnou společnost, BUT, Vnitřní projekty VUT, FIT-S-23-8209, start: 2023-03-01, end: 2026-02-28, running

Department of Information Systems (DIFS)